FSC Plotter: 0.143 and 0.5 resolution table for cryoSPARC FSC curves

Drop in a cryoSPARC FSC.txt export and get a table of the FSC 0.143 and 0.5 resolution crossings for every masked and unmasked curve. It can also plot the curves for figures. It runs in your browser as a single HTML file, so nothing is uploaded or installed.

Try it: https://ajitr66.github.io/fsc-plotter/

Resolution table

One row per curve: No Mask (unmasked), Spherical, Loose, Tight, Res, Auto-tight and Auto-tight corrected. Phase-randomized curves are included if you tick Include hidden columns.

Column	Meaning
0.143 first	Gold-standard (GSFSC) resolution, the value cryoSPARC reports
0.5 first	Conservative estimate, also used for map-vs-model FSC
last columns	Last crossing, which shows whether a curve dips and recovers

Values are in Å, linearly interpolated in frequency between the two shells either side of the threshold.

Copy table puts the values on your clipboard ready to paste into Excel, Google Sheets or a document. Export CSV saves them as a file. Both include the curves currently shown.

Plotting

• FSC curves with optional 0.5 and 0.143 threshold lines, and a cryoSPARC-style GSFSC Resolution title
• Preset color schemes, or Custom colors and line styles for each curve
• PNG and SVG export

Input

Both cryoSPARC export layouts work. If a file has only wave_number and no frequency column, you'll be asked for the box size and pixel size. The examples/ folder has one of each:

• fsc_with_frequency.txt: includes a frequency column
• fsc_wave_number_only.txt: wave_number only

Changelog

• v1.3.2: Precise x-axis labels keep trailing zeros, so every label shows 3 significant figures (12.0 Å, not 12 Å).
• v1.3.1: More space below the Copy table and Export CSV buttons.
• v1.3: Copy table and Export CSV for the resolution table.
• v1.2: Custom per-curve colors and line styles.
• v1.1: Reads wave_number-only exports. Labels are inferred from column keys, _raw columns are hidden by default, and there are x-axis tick options.
• v1.0: First release.

License

MIT, see LICENSE.