PoolMate

PoolMate is a browser-based equimolar pooling helper for planning library pools and dilutions, and generating the Eppendorf epMotion worklist CSVs (DNA.csv / Buffer.csv). Everything runs locally in the browser — no install, no server, and no data ever leaves your machine (entries are kept only in your browser's local storage).

▶ Use it now

https://akmartian.github.io/poolmate/

Open the link in any modern browser (Chrome, Edge, Safari, Firefox) on Windows, macOS, or Linux. Nothing to install.

What it does

PoolMate has five self-contained tasks, each with a live graphic showing where every sample goes and how much:

• Pool — combine libraries into one tube, or normalize each into its own well. Enter concentrations (ng/µL + fragment size, or direct nM, with replicate-read averaging) and a target (nM or fmol); PoolMate computes the equal-mole transfer per sample, the buffer top-up, and a post-rounding equimolar-deviation (CV%) check, then exports the epMotion DNA.csv and Buffer.csv. A per-sample share makes a pool deliberately uneven when you want it: 1 everywhere is equimolar, 2 gives that library twice the representation, 0 leaves it out.
• Normalize — dilute every library to one common concentration, plate → plate. Set a target in nM or ng/µL and a volume per well; each library can optionally be routed to a different output well (e.g. A3 → D5).
• Dilute — dilute one sample or a whole list. Tube view gives a virtual tube per sample with a Done checklist for bench work; Plate view gives a plate → plate map with per-sample in/out wells. Every parameter (target, final volume, min transfer, max capacity, rounding) has a shared default that can be overridden per row.
• Serial dilution — when a sample is too concentrated to reach the target in one step, the steps are laid out automatically. For pooling this is exported as an ordered Dilution.csv that runs first.
• Load — work out what to put on a flow cell. Pick Oxford Nanopore or Illumina and a flow cell / kit preset, enter one pool or several pools to combine (with a per-pool share), and PoolMate gives the µL of each pool plus the bench steps: the ONT loading mix (with the Elution Buffer top-up), or the Illumina dilution steps down to the loading pM. Presets are vendor starting points and every value is editable.
• Pool plan — every pooling run also writes a small <prefix>_pool.poolplan.json. Take it to the epMotion Protocol Builder (Pool tab → Import plan / CSV) and it writes a ready-to-run .export52 with the pre-dilutions, the pooling and the mixing in a single protocol — no worklist CSVs to load at the instrument. The builder exports the same format, and the Import CSV button here reads it back.
• Quality of life — platform presets (Illumina / ONT), run metadata, print / save to PDF, save & reopen runs as JSON, instrument CSV import, auto-named downloads, per-section and whole-page clear, and a fresh blank start each time (with one-click recovery of an unsaved session).

Dilution and pooling are always separate robot runs when you drive the instrument with these worklist CSVs: pooling must draw from wells that have already been diluted and mixed. A protocol built from a pool plan does both in one run, because it mixes each dilution itself before anything is pooled.

⚠️ The built-in platform presets are vendor starting points. Confirm all volumes and loading concentrations against your own kit / instrument SOP before running anything on the robot.

Repository layout

Path	What it is
index.html	The live web app (this is what GitHub Pages serves).
builder/	A page about the epMotion Protocol Builder, the companion program that turns a pool plan into a robot protocol. The builder itself lives in a private lab repository and the page links to it.
versions/	Archived snapshots of each working version (pool.html → pool_enhanced_v9.html → PoolMate_v10.html). The newest snapshot mirrors index.html and serves as a fallback.
Buffer.csv, DNA.csv	The original Eppendorf epMotion worklist templates this matches.
*.pdf	User guides.

Want to change it?

PoolMate is a single self-contained file (index.html — HTML, CSS, and vanilla JS, no build step). To customize it for your own lab, fork the repository and edit your copy; pull requests are welcome but not required.

License

MIT — see LICENSE.